In our laboratory we determinate phytohormones with HPLC-MS/MS and now we need to compare the routine technique with some new techniques that we found in different papers.
We need to determine the recovery of standard compounds in the new techniques.
Could anybody told me how to do this?
Dear Celeste, there is a useful flow diagram for you in Fig. 1, to plan out your phytohormone extraction procedures?
https://www.researchgate.net/publication/233324564_Analytical_methods_for_cytokinins?ev=prf_pub
Article Analytical methods for cytokinins
Dear Celeste, if you are dealing with plant liquids like coconut water, xylem/phloem sap or fruit juices, here is a slightly shorter method:
https://www.researchgate.net/publication/5561343_Simultaneous_analysis_of_different_classes_of_phytohormones_in_coconut_%28Cocos_nucifera_L.%29_water_using_high-performance_liquid_chromatography_and_liquid_chromatography-tandem_mass_spectrometry_after_solid-phase_extraction?ev=prf_pub
Article Simultaneous analysis of different classes of phytohormones ...
- Badges
- Science method