WGS for gram negatives. Better Illumina double read or illumina single read?

I work in gram positives (S. aureus) and lately We have done WGS illumina single read for different clinical and lab strains. The results are really good. Also I had seen publication with...

10 November 2017 3,549 2 View

What is the maximum amount of SNPs that a strain had to have to be consider isogenic with other?

05 June 2017 495 3 View

HPLC mobile phase problem, can somebody help?

Hello! I am having a mobile phase problem in the HPLC-UV. Where are measuring peptidoglycan composition of S. aureus MRSA. We run the samples and the buffer used for the samples as a...

04 May 2017 3,849 9 View

Can anyone help me with some techniques for phytohormones extraction for HPLC-MS/MS?

In our laboratory we determinate phytohormones with HPLC-MS/MS and now we need to compare the routine technique with some new techniques that we found in different papers. We need to determine...

08 September 2013 3,345 2 View

Method to extract and quantify polyphenols by HPLC-MS/MS?

I would like to know the different solvents and their concentration used in HPLC.

03 April 2013 3,774 6 View

Can anybody help me with a technique for measuring abscicid acid and its metabolites of shurbs?

the aim of my study is to measure ABA and its metabolites of shurbs from Patagonia Argentina (the shurbs is Larrea divaricata). My problem is that the leaves of this shrubs have a lot of resin...

04 May 2012 3,650 1 View

Is it possible that one single compound act as enzyme activator at low concentration but as an enzyme inhibitor when the concentration increase?

Can a single compound increase the enzyme activity at low concentration (below the known inhibition concentration) and when the concentration increase start to inhibit the enzyme? By the way, it...

01 January 1970 7,086 7 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 1,832 4 View