Can anyone help me with direct article on sex determination in spiders using flow cytometry?

More Chinmay Hemant Joshi's questions See All

Are there any genes in Stegodyphus spiders that are exclusively expressed in males or in females right from their birth(in spiderlings)?

I want to know whether there are any genes which are exclusively expressed in male and female spiderlings of Stegodyphus spiders (Or spiders in general).

04 May 2016 4,052 0 View

What methods can be used to determine sex of spiderlings of Stegodyphus sarasinorum just after hatching other than flow cytometry?

We tried to use Flow cytometry, but only single peak was observed and we are not able to resolve it

11 December 2015 3,647 7 View

Why there are (n-1) degrees of freedom for t-distribution or Chi-square distribution?

What does is mean physically to say that there are (n-1) degrees of freedom?

08 September 2015 6,317 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Olivier Catrice

What are the mechanisms of sex determination in spiders ?

Are there sexual chromosomes like in insects, birds and mammals ?

If so there may be a difference in the Genome Size/%GC between male and female.

To measure %GC you will have to use Hoechst 33342 (%AT) or Mithramycin/ Chromomycin A3 (%GC).

Problems come with particular excitation of these fluorochromes : 365 nm for Hoechst and Mithramycin, 458 nm for Chromomycin.

Nicole D Riddle

A Google search found this:

Flow cytometric sexing of spider sperm reveals an equal sperm production ratio in a female-biased species

What exactly are you looking for?