We tried to use Flow cytometry, but only single peak was observed and we are not able to resolve it
You could develop a PCR-based sex test using RAD-seq data.
See:
Gamble, T., and D. Zarkower. 2014. Identification of sex-specific molecular markers using restriction site associated DNA sequencing (RAD-seq). Molecular Ecology Resources 14:892–901.
Gamble, T., J. Coryell, T. Ezaz, J. Lynch, D. Scantlebury, and D. Zarkower. 2015. Restriction site-associated DNA sequencing (RAD-seq) reveals an extraordinary number of transitions among gecko sex-determining systems. Molecular Biology and Evolution 32:1296–1309.
Article Identification of sex-specific molecular markers using restr...
Article Restriction Site-Associated DNA Sequencing (RAD-seq) Reveals...
does this spider have a sex chromosome that can be PCRd for either male or female specific sequences ?
Thank you Tony and Paul for your kind replies. There is just a copy number variation in sex chromosomes i.e in males there is one copy of X1X2Y or X1X2o chromosome and in females there are two copies of the same.
I know nothing about spiders but the names of the chromosomes suggest that there is a Y sequence on one chromosome x1x2y but no Y sequence on the x1x20 chromosome in which case amplification of the y specific part like in humans would be definitive for males. Is this the case ?. Can females be any of x1x2y/x1x2y or x1x2y/x1x2o or x1x2o/xix2o ?
As far as I know, there is very limited data available regarding the sequence database of this spider, even I have not found anything much in the literature.
Do you think that anyone has done pcr sexing in a more common species of spider that has had its genome sequenced and that the genome might be sufficiently similar that the same gene/primers might be usable to differentiate your species. ?
Yes, there is one such study done. But in that study they have sequenced transcriptome of other species namely Stegodyphus llineatus and Stegodyphus tentoriicola. But still there is no information regarding any marker on sex chromosome. Following is the paper that talks about the same.
Article Orthologous genes identified by transcriptome sequencing in ...
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