Can anyone help me?? why after solubilization of decellular ECM, as soon as it placed in crosslin solution, it disintegrate?

More Saiedeh Erfanian's questions See All

Why does my protein precipitate after dialysis?

My protein forms a loose debris after dialysis, which reduces its performance. What can be done to prevent this?

03 June 2022 7,011 1 View

Are there any models (ideally based on microstructure) that can predict Lankford coefficients?

Hello researchers, In metal forming, as a result of cold rolling, grain shape is changed and elongated along the rolling direction. This results in anisotropy in the mechanical properties of a...

06 November 2021 8,445 8 View

Can I help me to find some sorce Stochastic integration operational matrix for Haar wavelet?

I need to solved function B(t) but I have not

16 January 2018 3,256 4 View

What statistical analysis can be applied on biodiversity results?

After measuring diversity indices (like shannon index or Simpson index), what analysis should be used to compare these indices between different subgroups? I think ANOVA and PostHoc tests should...

11 January 2015 2,891 28 View

How can I find raw microsatellite data from published articles?

I need some raw microsatellite data from a published article for my lecture on Population Genetics softwares, but I can't find anything.

03 May 2014 2,646 4 View

Can plant-plant communication occur under ecologically realistic conditions?

Plant-plant communication. Does it have any real benefits?

16 January 2014 2,718 15 View

What is the best way to measure species diversity in aquatic systems?

Number of individuals is essential for measuring species diversity, it is hard to count it for each species in aquatic systems (specially for algal species), what is the solution for this problem?

20 December 2013 7,140 7 View

What is the temporal ecotone?

Seasonal changes in a specific community can be regarded as a temporal ecotone?

12 November 2013 1,946 13 View

Perform Mantel test in Arlequin?

I'm trying to carry out the Mantel test using Arlequin, has anyone here done it before?

30 August 2013 5,388 6 View

Does anyone have experience with working on a species with taxonomic problem?

When I blast my sequences to gb, they are 99-95% identical to two different species one of these two species is the sister taxon of my hypothetical species, and the another one is the sister of...

04 August 2013 5,526 3 View

For the process of preparing decellularized matrices what is the ideal solution in which we can bring the tissue from slaughter house to lab?

I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...

31 July 2024 5,645 2 View

What are some signs that an ECM is overfitting the Nyquist plot?

What can you look for to know that the circuit model being used is overfitting the data. How do you know when overfitting is occurring?

31 July 2024 7,425 0 View

How do regenerate Amberlite XAD 4 resin for further cycles of use for removing 0.5% Sarkosyl from recombinant protein solutions?

I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...

25 July 2024 5,001 2 View

Regarding solubility of Dorsomorphin in DMSO ?

I'm using Dorsomorphin (C24H25N5O) as one component in dual SMAD inhibition of pluripotent stem cells to differentiate into neurons alongwith SB431542. Now as to solubilizing 10mg SB in DMSO for...

11 July 2024 4,832 0 View

Can Invitrogen™ LIVE/DEAD™ Cell Imaging Kit (488/570) be used on fixed cells?

Can the kit from Invitrogen™ LIVE/DEAD™ Cell Imaging Kit (488/570) be used on fixed cells on a scaffold?

08 July 2024 7,914 3 View

"What is the best method and model for evaluating the mechanical and viscoelastic properties of a viscous material with multiple particle inclusions?

"What is the most accurate method and model for evaluating the effective mechanical and viscoelastic properties due to the inclusion of multiple types of particles in a viscous material, and which...

16 June 2024 9,139 1 View

What are the conditions for using the ARDL model?

I have used the ARDL model to model data over ten years. The results of the bounds test indicate that there is a cointegration relationship at the 5% and 10% levels. However, the ARDL and ECM...

07 June 2024 8,818 4 View

How to solubilize Chitosan( high molecular weight) in a aqueous solution ?

Hi everyone, there is my issue: I try to synthetize nanoparticule of Chitosan according with this study doi: 10.1016/j.colsurfb.2011.09.042 I try it with HMW Chitosan (340-360kDa) but when I put...

29 May 2024 1,763 2 View

What is the identity of this E. coli protein that ends up in the insoluble fraction/ inclusion bodies?

I am purifying different proteins that go into inclusion bodies when expressed in E. coli. When I purify my inclusion bodies I always find two bands around 37 kDa in my solubilized fraction. These...

06 May 2024 6,324 2 View

How to distinguish between new collagen fibers and old ones in an ECM gel using Masson's trichrome staining?

hello, I have a scaffold with three layers, two synthetic polymer layers and one central layer made of ECM gel seeded with SMCs. After in vivo culture, how can I determine through Masson's...

05 May 2024 2,297 0 View

Wonil Han

What kind of tissue did you use? Increasing pepsin concentration would work, about 1.5-2 times higher. It could change physical properties, but it will not be very significant.

Saiedeh Erfanian

Thank you I really appreciate your help.

I used skeletal muscle tissue for decellularization.

The tissue/pepsin is 10 to 1 in most articles

Is it necessary to reduce the incubation time by increasing the concentration of pepsin?

thanks@