When I blast my sequences to gb, they are 99-95% identical to two different species
one of these two species is the sister taxon of my hypothetical species, and the another one is the sister of the clade which composed of my species and it's sister taxon, how can I prove my species is my hypothetical species?
ITs is my marker and i think my species is Juniperus polycarpos, but in Iran every taxonomists think this species is Juniperus excelsa , my sequences are similar to Juniperus excelsa and Juniperus procera, with same percentage.
There is a big difference between 99% and 95% sequence similarity. Also, the database of sequences you are Blasting against may not contain a complete set of all the relevant Juniperus taxa. According to the Genbank taxonomic list (http://www.ncbi.nlm.nih.gov/nuccore/?term=txid884040[Organism:exp]), there is no ITS sequence for J. polycarpos. So maybe you are right. You might contact the people who published the other genes from J. polycarpos and see if they can send you some DNA so you can compare it to your own sequences.
It's very good idea to contact people who working on this species and i try this job two times. unfortunately, there is no answer form them.
with my data, is there any method for detect and introduce J. polycarpos?
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