Could the "incoming radiation (net radiation)" values be negative?

Could the "incoming solar radiation" values be negative? What is the difference between "global solar radiation" and "incoming solar radiation"? Which one might be measured negative values?

21 February 2021 7,726 9 View

How long does it take for MCF-7 cancer cells to adhere to the microfluidic surface? Is 24 hours enough?

Hello everyone. We have cultured MCF-7 cancer cells in microfluidics several times and incubated them for 24 hours, but as the nanomaterial passed through the cells, the cells moved and no cell...

05 September 2020 7,292 3 View

Should Quartz powder or Sand water absorption be considered for UHPC mix design calculations? How is the water absorption of the powder calculated?

07 August 2020 1,592 1 View

What does scale.factor do exactly in LogNormalize function in R and why it is 10000 by default?

Hi, In LogNormalize function feature counts for each cell are divided by the total counts for that cell and multiplied by the scale.factor prior to log transformation. My question is what does...

26 July 2020 1,980 2 View

How to use all of CPU capacity for Analysis in Ansys mechanical Apdl?

Hello. Im using Ansys mechanical Apdl for timehistory analysis. I want to use all of Cpu's cores in this process. Help me please to do that with your guidance. #ansys #cpu_capacity

23 June 2020 7,600 3 View

How to simulate a porous media which is opaque to thermal radiation in Fluent Ansys?

Hello I am trying to model a porous zone in Fluent (the fluid in void is air) which is opaque to thermal radiation. If I set it as a porous zone with air as the media, Fluent treats it as a...

31 May 2020 7,450 2 View

Critical thinking assessments?

i'm trying to find a comprehensive explanation of most -if not all- of the instruments proposed to assess critical thinking skills and abilities. i will appreciate if you could introduce them or...

29 May 2020 5,717 5 View

How can I merge a slope raster to Land use-HSG shapefile in order to produce Curve Number in GIS?

Hi every body: I'm trying to calculate Run off Curve Number in GIS, first of all I need to merge my Land use-Hydrological soil group shapefile with Slope raster of my study area to produce CN...

19 April 2020 4,587 3 View

Does the presence of clones on the agar plate mean that the cloning has been done successfully?

I did cloning. it was like a traditional approach. Firstly, Restriction digestion was performed for both vector (8kb) and target gen (700bp), and also the vectors and target gene were run on the...

21 March 2020 9,618 4 View

What is the role of the fractal dimension(FD) in the analysis of a dynamical system such as Russler's?

According to the articles, we use fourier transform to compute power spetra density(psd) in spectral analysis , Sm = psd = lim {2|Ym|^2 /( N * delta) }, m=1,2,3,..., N / 2 psd is a function of...

03 March 2020 2,318 4 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Is there a way to extract total RNA and protein from cell culture at the same time, without denaturing protein?

Hi all, Is there a way to extract RNA and protein from the same cell culture sample? Because we need to use the protein for gelatin zymography at later time, we are looking for a method that can...

22 February 2021 5,341 5 View