Google is your friend, online there are lot of articles, this one is from nature protocols (http://www.nature.com/nprot/journal/v2/n2/full/nprot.2006.478.html)
Check another one from experimental biosciences. (http://www.ruf.rice.edu/~bioslabs/studies/mitochondria/mitoprep.html)
If you can afford kit for isolation of mitochondria, here it is.
Mitochondria Isolation Kit for Tissue (ab110168) (http://www.abcam.com/mitochondria-isolation-kit-for-tissue-ab110168.html)
Well, we have used a commercial kit available from Biovision Labs USA. The product is economical and has a user-friendly protocol. We have a couple of publications in which this particular kit was used. Please refer to the kit Technical Data Sheet and follow the instructions for tissue samples.
Article Occult hepatitis C virus elicits mitochondrial oxidative str...
Data Role and clinical significance of lymphocyte mitochondrial d...
The above answers are very adequate. We isolate mitochondria on a routine basis from many tissues including liver. But the key questions are: what do you want the mitochondria for and how pure do you need them.
thanks for your contribution , actually virgilio sir, i want to isolate mitochondria by normal manual method , because we cant afford kit for that and check enzyme assay of electron transport cycle .
This paper provides you with the protocol for the isolation method for several tissues, including the liver and it was the basis of the protocol in our lab.