I am growing mouse bone marrow macrophages in different synthetic scaffolds intended to support directed tissue growth and wound healing. We have shown that differences in our materials constrain many aspects of macrophage biology.
This question is now mainly about comparing glycolysis and oxidative phosphorylation, though I will be happy to hear about any assays that can be performed on conditioned media and that relate to cellular metabolism.
There is now a great interest in the relatedness of polarized state to cellular metabolism in macrophages.
I would like to be able to assay metabolic state in cells growing in different versions of our materials which have been shown to differently constrain polarization. The materials are opaque and are formed into discs 1mm thick and 6mm in diameter. It would be ideal for me to assay conditioned media as that would allow multiple time points from the same samples. Alternatively I can extract lysate from the cells within the materials for 1 time samples.
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