Stool examination while fresh. 58-year-old male subject with diarrhoea.
From my observations, there appear to be three principal avenues to engender critical thinking in education K-12: debate; mathematics, and science. For some students, all three avenues are...
04 February 2021 9,605 7 View
Hi, sorry I'm stumped. I'm going to do a study on the leadership styles (using the MLQ Form 5X) of minority women administrators in medical schools. How will I calculate the sample size? Thank...
30 January 2021 2,865 4 View
I want to process a very large file, let's say 300 GB, with Python and I'm wondering what is the best way to do it. One limitation is that my algorithm needs the whole file in memory, so a...
15 January 2021 6,691 4 View
Hi. I am doing an agarose gel electrophoresis. After running PCR samples in a 2% agarose gel at 90V x 45' I obtained the results in the image. The ladder bands are not properly stained. For...
14 January 2021 2,316 4 View
I would like to know if the properties of a polymer impact on the size of the particle it forms. I'm working with different polyssacharides (alginic acid from different seaweed), which are...
29 December 2020 7,333 6 View
I have an experiment where 10 groups of 3 judges are asked to evaluate a list of pictures in 2 evaluation conditions. The pictures were made in 2 between-subjects conditions (A,B) and each group...
20 December 2020 7,697 3 View
I am doing a bit of research on different areas (AI, cognition, music psychology, aging...) and have gathered so far a huge amount of information in the form of web links, pdfs, printed books and...
15 December 2020 8,004 5 View
Hi all, I am design now a liquid high pressure flow system to be used up to 50 bar, and I am having some doubts concerning how I can estimate the pump power. Besides the pressure drop in the...
15 December 2020 8,411 1 View
Hi all, I am trying to design a membrane system to remove the CO2 from a flue gas current, and I am having difficulties in finding information. Does anyone have any technical information,...
15 December 2020 3,655 2 View
Teleworking works best in terms of productivity and innovation if carried out in co-working, as the critical peer review allows to continuously improve the "deliverables"
03 December 2020 7,376 5 View
Extraction of DNA from faecal sample
16 August 2020 9,250 1 View
What would be the acceptable CV% and SD for copy number results between stool samples in qPCR (same group)? I am evaluating different bacteria (E.coli, Lactobacillus, etc.) using the absolute...
06 August 2020 8,112 4 View
Due to the pandemic, I can't go back to my university to conduct experiments. I have my stool samples and extraction kit with me in a smaller laboratory however they don't have the equipment I...
07 July 2020 5,172 1 View
After dilution of Fecal matter in PBS, the technician used the Noregen Urine Exosome Purification kit and RNA extraction. So my basic question is: Do you think we've got actual exosomes or just...
13 January 2020 1,420 2 View
The idea is to use rodents and to induce HGT within their microbiomes, one high exposure group, one low exposure and one control. The extent of HGT will then be assessed. What size would my sample...
31 December 2019 7,859 2 View
31 December 2019 7,685 2 View
I've joined a new lab, and my PI just told me that they have human fecal samples on swabs that have been stored at 4 degrees Celsius for about two years. She asked me if I thought it would still...
10 November 2019 158 3 View
I am working with DNA extracted from feces. I made a qPCR using concentrations of 100, 75, 50 and 20ng final. The CT value was very similar between dilutions between 29 and 30. My positive control...
10 November 2019 7,803 8 View
I want to isolate DNA and RNA from feline faecal samples in order to determine which pathogens (viruses, bacteria, fungi, parasites...) are present in the samples. The isolation will not be...
22 September 2019 3,029 4 View
I want to isolate DNA and RNA from faecal and blood samples in order to determine which pathogens (viruses, bacteria, fungi, parasites...) are present in the samples. I currently have one PCR...
08 September 2019 6,363 4 View
