Can anyone help me getting the protocol for separation of bacterial DNA from mammalian DNA mix using sucrose gradient method?

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Recently I am struggling to purify the full-Length human PARP1 using both pet28a and pet21b vectors. If anyone is getting good yield, protocol please?

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What are some key criteria for the computer system used in analyzing Whole Genome Sequencing data set?

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How can we disaggregate daily precipitation to hourly?

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How is damping ratio incorporated for bilinear isolators using elastomeric bearing plasticity element in openseespy ?

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Is there any method to extract building polygon shapefile of some historical timeline (Digitization is not an option as the spatial scale is large)??

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Modal Analysis of Base isolated structure in MATLAB and ETABS software not agreeing ?

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Is there a problem with my RNA pellet?

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Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

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RNA Extraction Using Hot Borate Method No Longer Working?

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Houda Kawas

Dear Mitesh Shrestha

Pls. find the attached files

Hoping this will be helpful to your project, good luck

Best regards

Prof. Houda Kawas

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3816399/

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC45776/pdf/pnas01101-0119.pdf

http://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=3&cad=rja&uact=8&ved=0ahUKEwixuqKEzMvTAhVHXiwKHZiZBmgQFggzMAI&url=http%3A%2F%2Fwww.nature.com%2Fprotocolexchange%2Fprotocols%2F1185&usg=AFQjCNGeJ8NSsyRykeEbb8_4pwuWXcZXHQ

https://www.qiagen.com/us/resources/molecular-biology-methods/dna/

http://scholar.google.com/scholar_url?url=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC389671/pdf/pnas00088-0154.pdf%2520sivu%25201&hl=ar&sa=X&scisig=AAGBfm2CtrngGOppCuewfNu-8JaIS89D5g&nossl=1&oi=scholarr

https://www.staff.ncl.ac.uk/p.dean/quiagen_molecular_biology_bench_guide.pdf

http://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=3&cad=rja&uact=8&ved=0ahUKEwjum4OW0svTAhVMiCwKHdYbAXgQFggzMAI&url=http%3A%2F%2Fwww.nature.com%2Fprotocolexchange%2Fprotocols%2F1185&usg=AFQjCNGeJ8NSsyRykeEbb8_4pwuWXcZXHQ

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC389671/pdf/pnas00088-0154.pdf

http://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=9&cad=rja&uact=8&ved=0ahUKEwixuqKEzMvTAhVHXiwKHZiZBmgQFghnMAg&url=http%3A%2F%2Fwww.gelifesciences.co.kr%2Fwp-content%2Fuploads%2F2016%2F07%2F023.6_Cell-Separation-Media.pdf&usg=AFQjCNGp2n9GWJy5iThYzbOZ_r_8QwjL0w

http://link.springer.com/protocol/10.1385/0-89603-127-6%3A235

http://download.bioon.com.cn/view/upload/month_0902/20090219_883c8288875809d32b83WmsR56xIB531.attach.pdf

Hoping this will be helpful to your project

good luck

Laith Al-Ani

271

Hi Mitesh

You can read some useful references 1

https://www.sciencedirect.com/science/article/pii/S0944501314001153

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC91166/

https://thermofisher.co.nz/Uploads/file/Scientific/Applications/Equipment-Furniture/Practical-Techniques-for-Centrifugal-Separations.pdf

https://www.nature.com/nprot/journal/v2/n3/full/nprot.2007.58.html

https://link.springer.com/protocol/10.1385/0-89603-127-6%3A235/fulltext.html

http://www.aun.edu.eg/molecular_biology/Proceeding_Dec2011/Current%20Protocols%20in%20Mol.%20Biol..pdf

http://www.sigmaaldrich.com/content/dam/sigma-aldrich/articles/biofiles/biofiles-pdf/biofiles_v6_n5.pdf

http://www.gelifesciences.co.kr/wp-content/uploads/2016/07/023.6_Cell-Separation-Media.pdf

Mitesh Shrestha

Thank you very much Houda Kawas sir and Laith Al-Ani sir..I am very grateful for providing me with the necessary articles..