Can anyone help me find a plasmid with a PH-sensitive GFP (preferably phluorin)?

02 February 2014 1 10K Report

I want to measure cytoplasmic ph, in E.coli, under several conditions in-vivo. To this end I would like to use cells expressing a plasmid harboring PH-sensitive GFP variant.

Does anyone have a plasmid like that and is willing to send it to my lab?

alternatively, can anyone please point me to a plasmid repository (other than 'addgene') that might have something like that in stock?

Hudec Roman

Hi Daniel,

you may try to write to Paulo Magalhaes from prof. Pozzan's lab in Padua Uni/Italy

maybe you can try directly thorugh their lab's page http://www.pozzanlab.org/group.php.

As I can recall correctly, Paulo was working on some pH-sensitiv GFP mutants. He is a very nice guy, and if it is possible he will help you.

Good luck

Roman

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