Daniel Zhitnitsky

2 Questions 10 Answers 0 Followers

Questions related from Daniel Zhitnitsky

Is there a method for creating E.coli spheroplasts without using EDTA?

I'm studying metal ions transport in E. coli, so the use of chelators like EDTA is somewhat problematic for my study. Does anyone know of a method without EDTA? Thank you.

07 July 2014 2,865 6 View

Can anyone help me find a plasmid with a PH-sensitive GFP (preferably phluorin)?

I want to measure cytoplasmic ph, in E.coli, under several conditions in-vivo. To this end I would like to use cells expressing a plasmid harboring PH-sensitive GFP variant. Does anyone have a...

02 February 2014 9,867 1 View