I am trying to take photos of H&E stained tissue with a monochrome Nikon microscope camera, and I can't seem to choose the right filter to apply to the image. I am using NIS Elements software. I would like to know does anyone have any suggestions if there is a pre-set H&E channel I am missing.
Hi, certainly you can take monochrome bright-field images by chosing an empty emission filter position and white light illumination in NIS Elements. However (to my understanding) b&w images of HE stainings may be of limited use. Maybe there is a technical solution to take sequential images with filters selectively blocking blue or red emission ans then assigning false colors (red and blue) to each channel. The composite image should somehow resemble a HE stain (OK..maybe thats a strange idea). The better solution may be to find alternative fluorescent dyes to H&E and acquire fluorescent images with your BW camera.
Since you are using a monochrome camera, the best you can do is pseudo color . Without a true color camera you will not be able to obtain realistic H&E stained images.
Thank you. I'm going to try and borrow another microscope for this part of my study.
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