Alexandra Tremblay

5 Questions 13 Answers 0 Followers

Questions related from Alexandra Tremblay

Why are my ODs the same on all my samples for ELISA?

I am running an ELISA on mouse serum that has worked beautifully in the past, and for some reason now it doesn't work for either the standard or the samples. I keep getting almost the same ODs for...

09 September 2015 6,848 6 View

Can anyone help me choose the right setting in NIS Elements software for nikon monochrome microscope camera for H&E stained tissue?

I am trying to take photos of H&E stained tissue with a monochrome Nikon microscope camera, and I can't seem to choose the right filter to apply to the image. I am using NIS Elements software....

07 July 2014 9,736 3 View

Polyethylene sheeting for heat sealer?

Fisher stopped selling their polyethylene heat sealer plastic, I was wondering if anyone had a reliable vendor who sells it/ or has more specifications on thickness etc. I am using it for western...

01 January 2014 6,524 0 View

Why does my NADPH oxidase ROS curve have two peaks when stimulating with PMA?

I am stimulating mouse bone marrow PMN with PMA in a luminol ROS detection system, and reading the luminescence with a luminometer for 1 hour. I always get two peaks in my curve (an sharp,...

06 June 2013 2,576 1 View

ELISA is broken?

I have run an ELISA that normally works to detect IL-6, but today (even with all newly made working reagents) the color change reaction is very very slow. Even the standard curve is very faint. I...

05 May 2013 3,067 34 View