I combined QCM with loop-mediated isothermal amplification (LAMP) for the detection of specific DNA. But the false positive result is big problem. Can anyone help solving this problem?
High sensitivity is a setback for LAMP. However, that sounds like contamination. Use fresh reagents, detect your products from a different place from preparation and other precautions for amplification.
I'm working with LAMP and the first time I had false positive as well, but I have solve it. It is really important use different place: A) reagents preparation B) Place where you mix the the sample with your reaction mix. C) Amplification / detection.
When you mix the sample with the reaction buffer is a good idea to use independent tubes (all closed).
Hello,
I agree with the answers above, I do the same to avoid false positive.
Through my days of screening the primer sets for LAMP, non-specific amplification can arise from the design of the primer set. I ran the isothermal reaction for 60 mins, and experienced this non-specific amplification coming up at typically 50-60 mins, sometimes none. It may be solved by fine tuning of the reaction temperature, but in my case I redesigned the primer set.
hope it would help
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