How to recycle Sodium Iodide?

Hi, I am using sodium iodide to separate density carbon fractions of my samples. I want to recyle it for further uses. While searching I only get the process to recycle sodium polytungstate (at...

20 September 2020 9,556 2 View

How does the pH of the solution affects the dispersion behavior of large sized graphene oxide nanosheets in various solvents?

I am trying to understand the dispersion behavior of graphene oxide in a range of organic and inorganic solvents to understand their specific applicability. Thus the pH dependent solubility...

26 August 2020 4,907 4 View

How to take PL of Methylammonium Lead iodide thin film?

I am working on methylammonium lead iodide perovskite thin films. Whenever I try to take PL of MAPbI3 thin films using Agilent Cary Eclipse Fluorescence Spectrophotometer I didn't get any peak....

11 April 2019 5,176 4 View

I am trying to subculture my isolated yeast strain from it's mother culture YPD medium plate. But its not growing at all. How to subculture it again?

I have tried with different media. But its not showing any result. Yeast strain Medium used: Yeast extract Peptone Dextrose Agar, Sabouraud Dextrose agar, Yeast extract Peptone Sucrose Agar.

06 December 2018 3,032 1 View

Which is the most efficient glass surface cleaning protocol?

I want to clean the glass surfaces for surface modification analysis, therefore i am exploring various glass cleaning protocols for the maximum possible decontamination. ( Organic, inorganic and...

29 August 2018 4,982 4 View

How can UV-Vis spectroscopy be used to calculate lateral dimension of 2D nanosheets?

I am trying to calculate the lateral dimensions of prepared graphene oxide nano-sheets dispersed in water, several articles have indicated that UV Vis can be used as a tool to do so . However, i...

19 February 2018 5,468 3 View

How the polydispersity of graphene oxide nanosheets synthesized by hummer's process can be minimized ?

I am preparing the GO nanosheets using Hummer's process, but synthesized nanosheets are of very high polydispersity in terms of lateral dimensions. Is there any method by which the polydispersity...

17 December 2017 7,229 3 View

Is it possible to segregate graphene oxide nanosheets from a solution on the basis of their lateral dimensions?

I have an aqueous solution of exfoliated graphene oxide ( by ultrasonication) , Now if i wish to segregate these nanosheets on the basis of their lateral dimesions, how can i do so.?

30 October 2017 7,789 3 View

Can AUVs compute Angle of Arrival of incoming signal in UWSN?

Are the Autonomous Underwater Vehicles (AUVs) capable of finding Angle of Arrival of an incoming signal?

12 May 2016 6,958 3 View

Can you make a clear differentiation between rheocasting and thixocasting processes?

11 September 2014 5,539 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View