Can anyone from India provide me cell lines.....

More Durbadal Ojha's questions See All

How method validation is done for any analyte in LC-MS/MS ?

I’d like to grasp the finer points of method validation and know which guidelines to adhere to. For pesticides, I understand the Sante Guideline is applicable, but what about mycotoxins such as...

07 April 2024 5,813 3 View

How to prevent my LC-MS/MS Columns from deteriorating ? How to recover a column by washing ?

Lately, I’ve been encountering issues with the longevity of my Liquid Chromatography tandem Mass Spectrometry (LC-MS/MS) columns. Specifically, I use the ZORBAX Eclipse Plus C18 RRHD (Rapid...

26 March 2024 5,129 6 View

Why dipole moment using bader charge and the cartisian coordinate is not matches with the given dipole moment in gaussian log file??

I am calculating the dipole moment (p) of a cluster using Bader charges (q) and cartesian coordinates (r) using the formula p =∑ q.r However, it is not matched when we match it with the Gaussian...

17 March 2024 6,612 0 View

Regarding query of LS-DYNA (dyn21umats.f)?

Dear all I am working on LS Dyna and searching for gauss points in the dyn21umats.f object file but I could not find it. I want to know if there are any common blocks , variables or any routine by...

05 August 2023 2,797 0 View

How a leakge inductance in Dual Active Bridge converter helps for power flow between two sources?

Current i am doing some research on energy storage application of dual active bridge converter. I am stuck with a question about the consequence that there might occur if the two voltage source is...

09 January 2023 1,535 1 View

Any good quality assessment tool for assessing quality of systematic reviews ?

Please suggest any good tool to evaluate the quality of systematic reviews for conducting systematic review of systematic reviews.Thank you in advance.

11 September 2022 166 4 View

What is the appropriate method of pellet count method for estimating spotted deer population ?

Can you suggest me any literature which suggests all the methods and procedures to obtain density/sq.km of spotted deer ?

05 September 2022 5,045 3 View

How do we distinguish Lesser Himalayan rocks from the Higher Himalayan and Tethyan Himalayan rocks?

The general notion is that the Lesser Himalayan Sequence (LHS) rocks are in the footwall of the Main Central Thrust (MCT), and the Greater Himalayan Sequence (GHS) thrusted over LHS along the MCT....

29 July 2022 1,153 2 View

How to properly search for patents with keywords on derwent?

While searching for patents on derwentinnovation.com I am facing a weird situation. While searching for patents with a certain set of keywords, I always use the 'smart search' option. Now while...

27 July 2022 5,131 3 View

How can we calculate the biomass of grass, leaves and buds?

what are the processes of biomass calculation? ? can you suggest me any literatures

22 July 2022 4,153 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

Saleh Alkarim

Hello

These companies (National Centre for Cell Science , Thermofisher) provide many cell lines in india

https://www.thermofisher.com/search/global/searchAction.action?query=cell+line&resultPage=1&resultsPerPage=15&autocomplete=

http://nopr.niscair.res.in/bitstream/123456789/6503/1/IJEB%2044(5)%20367-370.pdf

Poornima Sharma

even I need to purchase canine cell lines