Shan Sts

4 Questions 3 Answers 0 Followers

Questions related from Shan Sts

Can anyone explain how to design repair templates in the pSGKP-XX plasmid?

I am trying to use CRISPR and the Lambda red system for gene knockout in Klebsiella. I would like to seek advice on how to design the repair template. If anyone can advise me on this I highly...

06 May 2024 291 0 View

Hello,Can anyone suggest me protocols to do gene knockout in Klebsiella pneumoniae using CRISPR cas?

If anyone can advise me on any protocols which work best for Klebsiella gene knockout are welcome.

06 May 2024 2,743 0 View

Can anyone help me design gene knockout study for klebsiella ?

I am new to this concept and I need some guidance on designing the primers with restriction site, homologous region, and antibiotic resistance gene casseettee. I am also looking for the suicide...

04 March 2024 8,156 2 View

Hi, could someone please help me with gene knockout in Klebsiella (homologus recombination) and also I need suggestion for choosing the plasmid?

Thanks

29 February 2024 6,539 3 View