This is a 0.7% agarose gel. Left lane is (faint) 1kb ladder, next lane is a single cut, and huge right 'well' is double cut vector. 

I have digested about 40ug of plasmid with 2 enzymes, and compared it to about 1ug of singly cut plasmid. But both of them seem to be running kind of smeary. 

This was run at 110V for about 2hrs. My PI, having PI powers, has done essentially this same experiment, and has gotten clean bands... 

All of my other bands whenever run gels are crisp and clean (see reference image). 

Thanks in advance for any help!

David

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