David Ichikawa

2 Questions 2 Answers 0 Followers

Questions related from David Ichikawa

Can anyone diagnose the problem with my DNA gel?

This is a 0.7% agarose gel. Left lane is (faint) 1kb ladder, next lane is a single cut, and huge right 'well' is double cut vector. I have digested about 40ug of plasmid with 2 enzymes, and...

18 April 2017 9,715 3 View

After having too many colonies on my 'no insert' control plate, I treated with CIP and now have no colonies. Any trouble shooting suggestions?

I used a single restriction enzyme, so the first problem was likely religation of the same plasmid. I treated with 10 units of CIP (about 5X overage) for only 5 minutes at 37C, and now there are...

14 August 2016 8,349 13 View