Currently using diluted Hoagland's solution but suspect this is still too strong, looking for a nutrient solution for B.distachyon I can buy in bulk or easily make up.
It depends on your cultivation system. Do you use an aerated pure nutrient solution or are you cultivating in any type of soil or soilless substrate? You should control the electrical conductivity (EC) of your nutrient solution and of the rooting zone substrate in an acceptable range (electrical conductivity (EC) of approx. 1.5 dS m–1) to avoid osmotic stress.
In horticulture for fertigation (means continued fertilizer application with each irrigation event to the horticultural substrate) a concentration 0,15% nutrient salt w/v could be suitable...
(i.e.: in 1 l fertiliser solution 1.5 g solid salts comprising all nutrient salts of the Hoagland's solution has to be solved).
Helpful might be the following paper: Journal of Horticultural Science and Biotechnology 83 (2), 207-217
Hoagland solution is the best for almost crops including cereals/grasses. Never use full strength. Start from 10% and slowly raise to 50% for rest of the experiment.
I agree with Zahoor Nazeer; Hoagland's is good enough. There was some work in Texas on a specific grasses nutrient solution, but for general studies, diluted Hoagland' is a good benchmark.
I advise humic materials for this purpose.Humic material can be extracted from soft brown coallike deposits called leonardite, found with lignite coal. humic acid is widely sold and used without as much detailed research. This brief review will cover the nature of humic substances and how they are currently being used in crop production.
Humic materials can be easily extracted from vermicompost by using NaOH or KOH. Vermicompost can be produced from the post harvests and from animal dung (ruminants). I have grown paddy (Oriza sativa) without water logging conditions using vermicompost only in the reqular paddy growing areas. By use of vermicompost there was 20% less yield compared to chemical fertilization but the seeds were bold enough to fetch more price in the market and the mill owner was happy to get less wastage. Additionally we could find out that 5 acres of paddy could be grown by the same quantity of water utilized under stagnation of 1 acre.
I have seen people using 1/2 to 1/5 strength Hoagland solution for cereals and leafy vegetables with goog result. As suggested by someone, if you cultivate in pure nutrient solution, it is important to provide constant aeration and monitor the solution pH.
For hydroponic culture we have used a modified Hoagland's with good results:
Hydroponic plant growth
Seeds of Bd21-3 inbred line were sterilized in 15% bleach plus 0.1% triton X-100 for 30 minutes. After soaking in the bleach solution the seeds were rinsed with sterile distilled water {Huo, 2006 #4}. Sterilized seeds were cold treated at 4°C for seven days in order to synchronize germination. The seeds then were germinated on moistened clay balls (Hydroton) in mesh pots. When seedlings emerged, the pots were placed in a semi-aeroponic culture set up in which modified Hoagland’’s medium (1 mM KH2PO4; 3.75 mM KOAc; 5 mM Ca(NO3)2; 1.25 mM KNO3; 2mM MgSO4; 3.75 M NH4OAc; 46 μM H3BO3; 9.1 μM MnCl2; 0.77 μM ZnSO4; 0.32 μM CuSO4; 0.83 μM H2Mo4; 100 μM FeSO4; 100 μM EDTA) was continuously sprayed onto the growth substrate. Nutrient solutions were changed every two days. For the first two days, plants were grown in half-strength nutrient solutions in order to avoid root burn in the young seedlings; after two days, full-strength nutrient solutions were used.Plants were grown at 20°C with 20h of light at 200μE, and 4h dark in controlled growth chambers.
As described in: Yordem, B. K., Conte, S. S., Ma, J. F., Yokosho, K., Vasques, K. A., Gopalsamy, S. N. and Walker, E. L. (2011) Brachypodium distachyon as a new model system for understanding iron homeostasis in grasses: phylogenetic and expression analysis of Yellow Stripe-Like (YSL) transporters. Annals of Botany, 108(5): 821-835.
I am importing organic liquid nutrients from Australia from a company called Growgreen Technologies and marketing in India. They have products specific for supplementing specific nutrients. We have tried in different combinations for golf grasses, Paddy, plantation crops and some 20 different types for vegetable plants. Everywhere we got very good result. Some of their products are;
Profert - Processed from select aquatic animals using their proprietory microbes.
Microbe Plus - Essential Microbes with high levels of NPK and other micro minerals
Phoscal - Phosphorous and Calcium with live microbes
You may try them. If you need to know more about their products, please visit their website: www.growgreen.com.au
Thank you all so much for the replies, am currently diluting hoagland's, (1:5), it is to go into a rockwool cubes (although I have no idea what this already contains), and guess I will continue to use this as it seems to be having no negative effects :)
In hydroponics I've used 1-8 dilutions of Hoagland's with constant aeration and it worked well. Also used the rockwool cubes and changed the solution every other week.
Arnon and Hoagland' s solution is suitable for growing cerals. However you have to start with 1/4 th strength and you can increase 1/2 strength after 15 days . in case of maize you can increase 3/4 th and even full strength thereafter .
Hoagland's and similar solutions are unnecessarily concentrated; they can generate considerable osmotic stress or specific-ion toxicity if they are allowed to concentrate further by evaporation. As did the Letcombe group way back, and Arnold Bloom also, we have used extremely dilute solutions in flow conditions (J. Exp. Bot. 44:41-51 and 46:995-1009), but I imagine you don't want to do that. Still, you might want to flush and renew, which is always desirable - if you simply add new solution, you can get a buildup of nutrients that are taken up in a lesser stoichiometric ratio than other nutrients. This can lead to osmotic stress, or, at least, luxury consumption that tweaks the physiology. It would help to know the goal of your study, which, presumably, is not nutrient dynamics, or else you would need to design a custom solution and custom flow or replenishment system. I'm ready to respond further.
Hi, Lon, original Hoagland solution is veyr suitable for cererals in hydropoinic. Only one point: try to not changes it comoletelky during re-freshing, but keep at least 30% of the old one. It will help to axod stress during re-freshing! Good luck!