I have a heterogeneous culture of nitrifying bacteria that was originally isolated from the field.
It has been kept alive for several months in a bioreactor.
I want to pull some of this bacteria out of the bioreactor and put it in a fridge for several months to examine later and possibly use it to re-seed a different bioreactor.
What would be the best way to preserve a heterogeneous culture of nitrifying bacteria?
(As a note, this culture contains ammonia oxidizing, nitrite oxidizing, AND heterotrophic bacteria)
In our lab we coonserve the bacterial and fungal culture in microbank at -80°C
1. Refrigeration is useful at 0-4°C either in refrigerators or in cold-rooms for short duration preservation (2-3 weeks).
2. Preservation by overlaying cultures with mineral oil or liquid paraffin and which is stored preferably at 0-5°C for long term preservation (5-10 years). This technique is found useful for preservation of Nitrogen–fixing bacteria like Azotobacter.
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