No amplification of SSR markers on Pecan nuts project?

Hi all, I am doing an SSR project on Pecan nuts and I am struggling to get amplification on my PCR. Here is a summary of what I have done so far 1. DNA extraction was a modified CTAB protocol...

19 April 2020 8,678 2 View

By how much does the relative response factor of two isomers differ?

08 February 2017 2,178 3 View

Hi all, could anyone give me some advice on transformation via electroporation?

23 November 2014 3,510 3 View

Can fMRI be used to demonstrate telepathy?

I have an idea for an experiment requiring fMRI, which would demonstrate the existence of mirroring neuronal activity in the absence of visual or auditory stimuli. I would be interested in...

29 June 2014 5,990 6 View

Background and spots in Western Blot membrane?

Do you know why my membrane looks like this? I did this western for Anti-hnRNP D/AUF1 antibody ab50692, as primary antibody in 1:1000 overnight at 4c and Anti-rabbit IgG HRP linked antibody...

07 February 2021 5,591 4 View

IHC-P staining of the viral infection in the mouse pancreas?

Hi, I'm trying to stain a viral infection of the pancreas in paraffin sections of adult (3 month old) BALB/c mice. Prior to starting my histology analysis, I have titrated the organ to confirm...

10 December 2020 2,198 3 View

Why use STOP solution in ELISA?

At the last step for data analysis, we always use a STOP solution to stop the TMB oxidized by HRP. Why do we have to stop the reaction? Couldn't we measure the color wavelength of...

17 November 2020 5,177 3 View

Help regarding free-floating immunohistochemistry on mouse spleen?

Hello fellow researchers I am trying to stain cryostat-sectioned spleen tissue, using free-floating chromogenic immunohistochemistry. Unfortunately, I'm not able to detect any staining (MHC class...

13 November 2020 6,884 3 View

Anyone familiar with antioxidant studies on rats' brain homogenate?

I am looking for a skilled personnel in Kuwait University that is familiar with antioxidant studies using ELISA kit or other techniques on rat's brain homogenate, particularly the hippocampal and...

04 November 2020 5,481 1 View

ELISA plate losing colour?

I did a few ELISAs 3 days ago (six 96 well plates) and some of them (about 10) were positive. My detector was an HRP conjugated antibody and I used TMB substrate. I stopped the reaction with...

18 October 2020 8,234 2 View

Should absorbance be between 0 and 1 for ELISA?

Hello, I am running a sandwich-type ELISA for which the readout is absorbance at 450nm. The ELISA measures protein binding which I am screening for compounds to inhibit. The 570nm...

05 October 2020 9,813 6 View

How to quantify relative amounts of a substance with immunostainings through confocal microscopy when samples have high autofluorescence?

I am working with plant tissues, buds specifically, fixed in glutaraldehyde and embedded in LR White resin. I am working with the primary antibodies LM19 and LM20 targeting pectins. I am using a...

21 September 2020 5,213 6 View

Could I use 2ndry Ab (Goat Anti-Rabbit Immunoglobulins/HRP) several times in detection stage of WB once it is diluted in 5% BSA or milk/TTBS ?

Dear friends I am optimizing WB on liver tissue collected from mice and I need an answer for above mentioned question according to your expertise?

07 September 2020 1,607 3 View

How do I get rid of noise in my western blot?

02 September 2020 5,038 2 View