Cannot open big files in GraphPad Prism?

Good morning. I have recently switched computers and gotten a new edition of Graphpad. I am trying to open a very large file containing all of my graphs for a project, but am encountering an...

22 March 2020 6,810 1 View

Sorting negative cells from GFP dim parental, please help?

I have an established cell line that expresses GFP constitutively. Once I transfect the cell line, there shouldn't any GFP expression since the DNA payload doesn't have a fluorescence marker. My...

23 September 2019 3,940 1 View

FACS-related Co-op project, can someone help me with their input?

Good evening - I need to come up with a project idea for co-op. The project needs to be mostly FACS-related but also give exposure to other techniques, such as cell culture and molecular biology...

18 September 2019 8,911 2 View

How do I run moderation with repeated samples and no experimental manipulation?

Hi everyone! I was wondering if anyone might have any ideas of how to conduct some potential analyses for some research I am working on. I am interested in the moderating role of trait emotion...

09 May 2019 5,118 3 View

How to link SAP2000 to matlab?

About connecting SAP with matlab

16 November 2018 2,663 3 View

How to link opensees to matlab?

I want to link matlab to opensees to complete the dynamic stability analysis of a 2D frame

13 August 2018 3,721 4 View

I want to measure the level of the tlr4 in tumor tissue, which method is better Immunohistochemistry vs western blot?

Dear all, Anybody could explain to me which method is better for measurement the level of toll like receptor protein in tumor tissue , Immunohistochemistry vs western blot? Thanks in advance.

09 April 2018 6,343 5 View

What is the pH to reach in measuring titratable acidity?

Dear all, For measuring the titratable acidity, I see titration of sample with 0.1 N NaOH to reach specific pH and reported by lactic acid. But the specifi pH I see is different in literiture,...

15 March 2018 8,141 3 View

Any tips for rodent sperm isolation/RNA extraction from sperm?

My lab is interested in extracting RNA from sperm (small RNAs specifically), and I believe we have the correct kit (Zymo Quick-RNA Micro-prep), but we already have a lot of trouble with extracting...

06 August 2017 8,639 1 View

Measuring prebiotic activity of a fruit

05 August 2017 5,723 5 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View