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Dear all, For measuring the titratable acidity, I see titration of sample with 0.1 N NaOH to reach specific pH and reported by lactic acid. But the specifi pH I see is different in literiture,...
16 March 2018 8,241 3 View
Hi everyone, I need to measure prebiotic activity of some samples, I read that SCFA is a good indicator of prebiotic activity of my samples because probiotic growth is encouraged and SCFA are...
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Hi everyone, I want to measure prebiotic activity of a fruit powder ( using fecal microbiota), I am wondering if I measure short chain fatty acids at the end of the test , how can I know that...
06 August 2017 5,854 5 View
I have made methanolic extract of a specific flower to use as antioxidant in frying, but the moment I added it to the hot oil, it burned, while many papers have used the extract in temperature of...
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Hi everyone, can anyone please tell me if we zero the blank in spectrophotometer in DPPH assay? or we record the number of blank and continue reading the number for samples without doing zero for...
15 July 2016 3,124 5 View
Different literatures read spectrophotometer in different time, I am very confused how and when to read the numbers of spectrophotometer and how to zero it. Please let me know if you can clarify...
13 July 2016 5,763 3 View
Confused about choosing which protocol among many protocols for different assays and this time about DPPH assay! please suggest me any good and well known DPPH protocol. And in general if you know...
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