Dear Long,

beta-SITOSTEROL is soluble in water and ethanol. See the following data on this agent.

PRODUCT IDENTIFICATION

CAS NO.

83-46-5

EINECS NO.

201-480-6

FORMULA

C29H50O

MOL WT.

414.71

H.S. CODE

2906.13.5000

TOXICITY

SYNONYMS

Stigmast-5-en-3beta-ol;

22,23-Dihydro-Stigmasterol; beta-Sitosterin; Angelicin; Cinchol; Cupreol; Rhamnol; (3beta)-Stigmast-5-en-3-ol; alpha-Dihydrofucosterol; Quebrachol; 24alpha-Ethylcholesterol; 5-Cholesten-24beta-ethyl-3beta-ol; Stigmast-5-en-3-ol;

SMILES

[C@@]12([C@H]([C@H]3[C@@H]([C@@]4(C(=CC3)C[C@H] (CC4)O)C)C C2) CC[C@@H]1[C@@H](CC[C@@H] ([C@H](C)C)CC) C)C

CLASSIFICATION

Phytosterol

EXTRA NOTES

β-Sitosterol is one of several phytosterols (plant sterols) with chemical structures similar to that of cholesterol. (wikipedia)

Other RN: 8003-23-4, 15764-35-9 76772-70-8 182512-23-8

PHYSICAL AND CHEMICAL PROPERTIES

PHYSICAL STATE

white solid

MELTING POINT

130 - 145 C

BOILING POINT

SPECIFIC GRAVITY

SOLUBILITY IN WATER

Practically soluble (soluble in ethanol)

pH

VAPOR DENSITY

REFRACTIVE INDEX

NFPA RATINGS

Health: 1; Flammability: 0; Reactivity: 0

AUTOIGNITION

FLASH POINT

STABILITY

Stable under normal conditions

EXTERNAL LINKS & GENERAL DESCRIPTION

USA.gov - beta-Sitosterol

Wikipedia Linking - beta-Sitosterol

Google Scholar Search - beta-Sitosterol

U.S. National Library of Medicine - beta-Sitosterol

PubChem Compound Summary - beta-Sitosterol

KEGG (Kyoto Encyclopedia of Genes and Genomes) -  beta-Sitosterol

http://www.ebi.ac.uk/chebi/ -  beta-Sitosterol

http://www.ncbi.nlm.nih.gov/ -  beta-Sitosterol

EPA - Substance Registry Services - beta-Sitosterol

Local:

Phytosterol: various sterols naturally obtained from plants. It is chemically similar to cholesterol which is obtained from animals. Sterol consists of three fused cyclohexane rings with hydrophenanthrene ring arrangement; the side chains vary sterols (more than 40 phytosterols are known). Beta-sitosterol is the most abundant phytosterol and others include campesterol, stigmasterol, ergosterol, brassicasterol, delta-7-stigmasterol and delta-7-avenasterol. They are waxy, clear solids soluble in most organic solvents but insoluble in water, and contain one alcohol functional group. Sitosterols are considered to play roles in lowering cholesterol in body (anticholesterolemic agent). stigmasterol is an unsaturated plant sterol occurring in plant fats like such as calabar bean, soybean oil, rape seed and cocoa butter. It is used as a starting material in the manufacture of synthetic progesterone, a female sex hormone that plays an important physiological role to regularize and rebuild  changes to the body caused by estrogen as well as in the luteal phase of the menstrual cycle. Its levels vary during the menstrual cycle.  In addition, progesterone is used as an intermediate in the biosynthesis of androgens, estrogens, and the corticoids. Synthetic compounds with progesterone are used in the prevention of miscarriage, in the treatment of menstrual disorders. Ergosterol, also called provitamin D2, is an unsaponifiable lipids; found in ergot, yeast, and other fungi; a white crystalline compound insoluble in water and soluble in organic solvents. It is converted to ergocalciferol (vitamin D2) upon irradiation by ultraviolet light or electronic bombardment.  vitamin D is either of two fat-soluble sterol-like compounds with antirachitic activity, or both collectively: ergocalciferol (vitamin D2) and activated cholecalciferol (vitamin D3). Ergocalciferol is formed from the irradiation of ergosterol found in dietary supplements such as fish liver oils, egg yolks, and fortified dairy products. However, cholecalciferol is normally synthesized in the skin, where ultraviolet light activates the compound 7-dehydrocholesterol. Vitamins D2 and D3 are about equal in activity in all mammals. Deficiency of vitamin D can result in rickets in children and osteomalacia in adults, while ingestion of excess levels can lead to hypercalcemia, mobilization of calcium from bone, and renal dysfunction.

SALES SPECIFICATION

APPEARANCE

white crystals

STEROL COMPOSITION

Phytosterols: 95% min

beta Sitosterol: 40% min

Stigmasterol: 15 - 30%

Campesterol :15 - 30%

OTHER INFORMATION

Hazard Symbols: , Risk Phrases: , Safety Phrases: 24/25-28A-37-45

Hoping this will be helpful,

Rafik

 Dear Long,

SOLUBILITY IN WATER Practically soluble (soluble in ethanol).

Good luck,

Rafik

Dear Long,

beta-SITOSTEROL is soluble in water and ethanol (see the following data):

PRODUCT IDENTIFICATION

CAS NO.

83-46-5

EINECS NO.

201-480-6

FORMULA

C29H50O

MOL WT.

414.71

H.S. CODE

2906.13.5000

TOXICITY

SYNONYMS

Stigmast-5-en-3beta-ol;

22,23-Dihydro-Stigmasterol; beta-Sitosterin; Angelicin; Cinchol; Cupreol; Rhamnol; (3beta)-Stigmast-5-en-3-ol; alpha-Dihydrofucosterol; Quebrachol; 24alpha-Ethylcholesterol; 5-Cholesten-24beta-ethyl-3beta-ol; Stigmast-5-en-3-ol;

SMILES

[C@@]12([C@H]([C@H]3[C@@H]([C@@]4(C(=CC3)C[C@H] (CC4)O)C)C C2) CC[C@@H]1[C@@H](CC[C@@H] ([C@H](C)C)CC) C)C

CLASSIFICATION

Phytosterol

EXTRA NOTES

β-Sitosterol is one of several phytosterols (plant sterols) with chemical structures similar to that of cholesterol. (wikipedia)

Other RN: 8003-23-4, 15764-35-9 76772-70-8 182512-23-8

PHYSICAL AND CHEMICAL PROPERTIES

PHYSICAL STATE

white solid

MELTING POINT

130 - 145 C

BOILING POINT

SPECIFIC GRAVITY

SOLUBILITY IN WATER

Practically soluble (soluble in ethanol)

pH

VAPOR DENSITY

REFRACTIVE INDEX

NFPA RATINGS

Health: 1; Flammability: 0; Reactivity: 0

AUTOIGNITION

FLASH POINT

STABILITY

Stable under normal conditions

EXTERNAL LINKS & GENERAL DESCRIPTION

USA.gov - beta-Sitosterol

Wikipedia Linking - beta-Sitosterol

Google Scholar Search - beta-Sitosterol

U.S. National Library of Medicine - beta-Sitosterol

PubChem Compound Summary - beta-Sitosterol

KEGG (Kyoto Encyclopedia of Genes and Genomes) -  beta-Sitosterol

http://www.ebi.ac.uk/chebi/ -  beta-Sitosterol

http://www.ncbi.nlm.nih.gov/ -  beta-Sitosterol

EPA - Substance Registry Services - beta-Sitosterol

Local:

Phytosterol: various sterols naturally obtained from plants. It is chemically similar to cholesterol which is obtained from animals. Sterol consists of three fused cyclohexane rings with hydrophenanthrene ring arrangement; the side chains vary sterols (more than 40 phytosterols are known). Beta-sitosterol is the most abundant phytosterol and others include campesterol, stigmasterol, ergosterol, brassicasterol, delta-7-stigmasterol and delta-7-avenasterol. They are waxy, clear solids soluble in most organic solvents but insoluble in water, and contain one alcohol functional group. Sitosterols are considered to play roles in lowering cholesterol in body (anticholesterolemic agent). stigmasterol is an unsaturated plant sterol occurring in plant fats like such as calabar bean, soybean oil, rape seed and cocoa butter. It is used as a starting material in the manufacture of synthetic progesterone, a female sex hormone that plays an important physiological role to regularize and rebuild  changes to the body caused by estrogen as well as in the luteal phase of the menstrual cycle. Its levels vary during the menstrual cycle.  In addition, progesterone is used as an intermediate in the biosynthesis of androgens, estrogens, and the corticoids. Synthetic compounds with progesterone are used in the prevention of miscarriage, in the treatment of menstrual disorders. Ergosterol, also called provitamin D2, is an unsaponifiable lipids; found in ergot, yeast, and other fungi; a white crystalline compound insoluble in water and soluble in organic solvents. It is converted to ergocalciferol (vitamin D2) upon irradiation by ultraviolet light or electronic bombardment.  vitamin D is either of two fat-soluble sterol-like compounds with antirachitic activity, or both collectively: ergocalciferol (vitamin D2) and activated cholecalciferol (vitamin D3). Ergocalciferol is formed from the irradiation of ergosterol found in dietary supplements such as fish liver oils, egg yolks, and fortified dairy products. However, cholecalciferol is normally synthesized in the skin, where ultraviolet light activates the compound 7-dehydrocholesterol. Vitamins D2 and D3 are about equal in activity in all mammals. Deficiency of vitamin D can result in rickets in children and osteomalacia in adults, while ingestion of excess levels can lead to hypercalcemia, mobilization of calcium from bone, and renal dysfunction.

SALES SPECIFICATION

APPEARANCE

white crystals

STEROL COMPOSITION

Phytosterols: 95% min

beta Sitosterol: 40% min

Stigmasterol: 15 - 30%

Campesterol :15 - 30%

Brassicasterol: 5.0%min

beta-SITOSTEROL

40.0% min

HEAVY METALS

20ppm max

ARSENIC

1ppm max

LEAD

none

ASH

1.0% max

MICROBIOLOGICAL TESTS

Total plate count: 1000CFU/g mac

Yeast and mold: 100CFU/g

Salmonella: negative

E.Coli: negative

Staphylococcus: negative

P.Aeruginosa: negative

MELTING POINT

130 - 145 C

Hello,

I believe the above answer is based on a misprint.  Beta-sitosterol is INSOLUBLE in water, not soluble as stated in that answer. However it is soluble in ethanol, and most useful, it is soluble in most vegetable oils

Beta-sitosterol | CasNO.83-46-5 - LookChem

www.lookchem.com › Products

Beta-sitosterol is insoluble in water while soluble in chloroform and carbon disulfide.

beta-Sitosterol | 83-46-5 - Chemical Book

www.chemicalbook.com › ChemicalBook › CAS DataBase List

Visit ChemicalBook To find more beta-Sitosterol(83-46-5) information like ... solubility : chloroform: 20 mg/mL, clear, colorless. Water Solubility : INSOLUBLE ...

Beta-sitosterol is soluble in Chlorofom.

you could try Glycerol : DMSO (1:9) rather than DMSO alone

Beta sitosterol is completely soluble in absolute ethanol at a concentration of 1mg/ml. (proved by performance)

Have you tried chloroform?

For animal experiment, please try suspending it in CMC as most of the solvents will have toxicity

Thanks

Beta-sitosterol is soluble in ethanol.

I recommend to try

1) sulfobutyl ether beta-cyclodextrin (SBE7-β-CD, SBE-β-CD, Captisol)

2) Heptakis(2,6-di-O-methyl)-β-cyclodextrin (DIMEB, DMCD)

They both solubilize cholesterol quite well as well as plenty of other hydrophobic compounds (including steroids), so I expect them to be good for this sterol. SBE-β-CD is essentially harmless but will bind less. DMCD will bind the most of sterol among cyclodextrins (e.g. 24 mg/ml in 30% w/v DMCD solution) but because of this it also has toxicity when injected or in cell cultures above certain low concentrations - due to the binding of choleserol from cell membranes. If your preparation of DMCD will be saturated with sterol, it will probably be well tolerated even at higher concentrations even when in IV or cell cultures.

References:

1. Article Evaluation of a Sulfobutyl Ether β-Cyclodextrin as a Solubil...

2. Article Cyclodextrin (CD) complexes of cholesterol - Their potential...

3. József Szejtli

Article Dimethyl-B-cyclodextrin as parenteral drug carrier

4. Article Fatty Acid-Cyclodextrin Complexes: Properties and Applications

5. Article Evaluation of the cytotoxicity of β-cyclodextrin derivatives...

It was good to learn from this thread (Matthew R Clark' answer) that sterols have a decent solubility in triglycerides (and thus in vegetable oils):

Article Effect of an aqueous phase on solubility of cholesterol in a...

Beta-sitosterol is sterol compound so it is not soluble in water but it is soluble in acetone, ethanol, carbon disulfide.

Can dissolve in Absolute (100%) EtOH up to 15 mM (approx. 6-6.5 mg/ml).

Ethanol

methanol

ethyl acetate

Acetone

n-hexan