How can I over express the active protease of silkworm in E.coli BL 21 (DE3)?

Hi…researchers….. I have cloned a mature serine protease into pET30a vector and tried to express in E.coli, BL 21 (DE3). I have followed the expression setup as given below: Day 1: grown overnight...

27 August 2015 7,212 4 View