Can anybody suggest how to do isolation and screening of Monooxygenase and dehydrogenase enzymes?

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Peter D G Dean

If you google 'NAD Sepharose' or Cibacron Blue Sepharose, you will find many dehydrogenases bind quite selectively to these materials. Adding the substrate to the elution step can enhance specificity. There is a huge literature on these so-called group-specific matrices. Good luck!

Shelley Minteer

After isolating the NAD-dependent enzymes, you will normally do spectroscopic activity assays to screen for the reactions that you want.

Praveen KUMAR S V

how to assay mono and di oxygenase enzyme assayin culture filtrate? Suggest some references.