It depend on what kind of study you want to perform? For e.g We use human neuroblastoma SH-SY5Y cell line as an in vitro model of dopaminergic (DAergic) neurons as this cell line posses many characteristics of DAergic neurons.

We are working on PVN in rat heart failure models.

what are the neuronal characteristic you are looking for ? I have seen couple of studies where they talk about PVN, they have used SH-SY5Y cells.

I would suggest PC12 cell-line. You could refer this review:

http://www.ncbi.nlm.nih.gov/pubmed/16946006

Thanks a lot

We work sometimes on F11 cells a freaky fusion cell line made of rat DRG-neurons and mouse neuroblastoma cells. They show neurite outgrowth, action potentials and express several neuronal markers as refered in PNAS by Platika et al. 1985.

Hello. Many options are available it depends on the research topic. Very easy to use and commonly employed are the human neuroblastoma cells SH-SY5Y. Rat PC-12 cells are also used as models for dopaminergic cells. If you are researching ALS NSC-34 are good option fot motor neuron-like cells. Good luck!

NT2 (N-tera) teratocarcinoma cells, upon retinoic acid induced differentiation to NT2-N, are used as a model of cortical neurons.

Anybody know of a good serotonergic human neuronal cell line?

i also faced the same question, i studied the apoptosis of motoneuron after spinal cors injury, is there anyone knows which cell line is suitable for our in vitro study?

There are several neuronal cell lines avaialble commercially - human neuroblastoma SH-SY5Y, two rat - PC12 and N27 cell lines and 3 mouse - MN9D, Cath.a and CAD cell lines. You need to choose depending on your work requirement.

All the best. Mohan

By the by, those cell lines (SH-SY5Y, PC12, N27, MN9D, Cath.a and CAD) I have just listed are dopaminergic in nature.

Mohan

Thank you, Alex, for answering my query. It is very helpful.

we use PC 12 cells which can be easily differentiated into neuron-like cells using FGF or NGF. We also used NT2 cells which are differentiated into neuronal like cells by retinoic acid, but in my opinion, this cell line is not very easy to grow in high amounts and require quite a long time to differentiate.

I just want to give you some more informations about NT-2 cells, you can consult Langlois A and Duval D differentiation of human NT-2 cells into neurons and glia: Methods in Cell Science 19 (1997) 213-219.

Several other cell lines have also been used in the litterature as model for neurons:

mouse hippocampal cells : HT-22 cells see numerous reports by Maher P and Schubert D

Human neuroblastoma cells either IMR-32 cells or SH-SY5Y.

It depends on what you want to study in theses models

which cell line is best for neural tissue engineering for treating Alzheimer disease