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Questions related from Mayur S Parmar
I am performing western blot analysis to detect the protein expression changes in the brain tissues from several neurodegenerative diseases (e.g Alzheimer's, Parkinson's) as compared to normal...
06 June 2018 603 8 View
I am trying to detect CD68 protein expression in mouse brain tissue. I have tried Anti-CD68 antibody [ED1] (ab31630) from abcam and it is giving me band at ~ 37 kDa, which abcam is suggesting....
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Would like to measure oxidative stress in the brain tissue. I am thinking to measure lipid peroxidation using MDA levels. If anyone has any suggestions, to measure oxidative stress in the brain...
01 January 2015 9,060 7 View
One of the research groups has shown that they are not able to detect ERK5 protein expression (taking into consideration proper negative and positive control) in brain using immunohistochemistry...
06 June 2013 9,579 28 View
A) Why would one prefer 1XTBST over 1XPBST or vice versa? B) If one is detecting phospho proteins, does it matter which one you are using? I have been doing Western Blots for a very long time...
05 May 2012 1,745 14 View
A) Do you agree this is the best stripping solution recipe : Preparation of 100 ml Stripping Solutions (Final Concentration = 100mM): 2 gm SDS 6.25 ml 1M TRIS [PH=6.7] Make it up-to 100 ml...
05 May 2012 7,460 53 View
A) Serum withdrawal prior to treatment / lower the serum concentration from 10% to 0.5 or 2.5% OR B) No serum withdrawal at all prior to treatment and perform treatment in regular media.
05 May 2012 6,421 5 View
