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Questions related from Mayur S Parmar
I am performing western blot analysis to detect the protein expression changes in the brain tissues from several neurodegenerative diseases (e.g Alzheimer's, Parkinson's) as compared to normal...
06 June 2018 603 8 View
I am trying to detect CD68 protein expression in mouse brain tissue. I have tried Anti-CD68 antibody [ED1] (ab31630) from abcam and it is giving me band at ~ 37 kDa, which abcam is suggesting....
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Would like to measure oxidative stress in the brain tissue. I am thinking to measure lipid peroxidation using MDA levels. If anyone has any suggestions, to measure oxidative stress in the brain...
01 January 2015 9,060 7 View
One of the research groups has shown that they are not able to detect ERK5 protein expression (taking into consideration proper negative and positive control) in brain using immunohistochemistry...
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A) Why would one prefer 1XTBST over 1XPBST or vice versa? B) If one is detecting phospho proteins, does it matter which one you are using? I have been doing Western Blots for a very long time...
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A) Do you agree this is the best stripping solution recipe : Preparation of 100 ml Stripping Solutions (Final Concentration = 100mM): 2 gm SDS 6.25 ml 1M TRIS [PH=6.7] Make it up-to 100 ml...
05 May 2012 7,460 53 View
A) Serum withdrawal prior to treatment / lower the serum concentration from 10% to 0.5 or 2.5% OR B) No serum withdrawal at all prior to treatment and perform treatment in regular media.
05 May 2012 6,421 5 View