We work with human TEC, so I unfortunately don't have a mouse protocol to give you, but I know that the Hogquist group published a detailed protocol with video in the Journal of Visualised Experiments, that could help you:

http://www.jove.com/video/51780/isolation-identification-purification-murine-thymic-epithelial

Thank you Christina.

This will work

Protocol for isolation of cells from lymph nodes

Starting material – isolated lymph nodes in PBS + 2% FBS

Protocol:

1. Put fresh 40 um cell strainer on 50 ml Falcon tube

2. Wash the strainer with 1 ml of PBS + 2% FBS

3. Put the lymph node on the strainer and push the cells through it using back of 1 ml syringe

4. Wash the cell strainer and the back of syringe with 2 ml of PBS + 2% FBS

5. Centrifuge the cells at 200 g for 10 min at 4°C and remove the supernatant

6. Resuspend the cells in 1 ml of PBS + 2% FBS and filter the cell suspension through 40 um cell strainer placed on 5 ml falcon tube