Can anybody provide me the protocol for pigment extraction from leaves for HPLC analysis

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M Moyen Uddin PK

You mean that pigment extraction from leaves by HPLC.

May be I am wrong.

If i am right, then you should go at http://prometheuswiki.publish.csiro.au/tiki-index.php?page=Determination+of+chlorophylls+and+carotenoids+by+HPLC

Ebenezer Idowu Ajayi

Pigment extraction will focus more on anthocyanins, right?

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3698059/

Bela Böddi

Dear Madhu Raina,

The answer to your question depends on your experimental plant. Ther second important subject is the purpose of your experiment. What pigments do you want to separate and study by HPLC. After clarifying these, you may get proper answers.

Best greetings,

Bela Boddi

William Letter

Madhhu: More information is needed to assist you. Which pigments? What exactly did you try and what results did you obtain? Please be detailed in your response.

Additionally, if you do a simple keyword search on the web using the specific pigment chemical names (or chemical group) plus "HPLC", then you will obtain many related papers and methods quickly (far faster than asking here).