Occasionally I get some non-specific reaction in ELISA. I belive that this colud be due to there may some antibodies for the carrier protein. To avoid the interferences in ELISA it would be better to use coating antigen should be different one than used for immunization. I have antibodies Aflatoxin B1-BSA conjugate and prefer to use Aflatoxin B1-KLH as coating conjugate in the indirect competitive ELISA. Looking forward for any comment or suggestions
Reddy, see attachement. The protocol described in the paper will give you the desired product though it is described for coupling to HRP. I used the protocol before and it was a success.
Hi Sv Reddy, here in our lab we use Aflatoxin B1 conjugated to HRP which is very specific. Maybe you could try HRP?
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