I want to stain the mitochondria in suspension cells by using mitotracker red (Invitrogen). Can anybody explain protocol to observe them in fluorescence microscope?
invritrogen also has good protocols for these markers. It's relatively simple, just add for 20-30 mins, change media and put under microscope. You'll have to play a little with the concentration depending on the cell type.
Note, that he wants to stain suspension cells. Dye uptake is the same, but the preparation of sample is a bit difficult. i have no experience with suspension cells under the microscope, sorry.