Ram Babu Undi

7 Questions 12 Answers 0 Followers

Questions related from Ram Babu Undi

Is it possible to validate a miRNA target in a test tube by providing all the essential factors, and how can we determine the half life of a miRNA?

To validate a miRNA target mRNA can we do it in vitro by adding everything in a test tube? If not, what are the difficulties and how can we overcome this?

04 September 2014 7,083 4 View

Can anyone explain how to measure mitochondrial copy number using realtime PCR approach?

I am using realtime PCR approach to measure mtDNA copy number with mitochondrial tRNA or rRNA and nuclear B2M primers. I isolated DNA from both the samples control and treated then I did real...

28 July 2014 3,170 6 View

Why is protein aggregating during concentration by amicon?

Buffer composition is 50mM tris pH7.5(p.I 6) 150mM Nacl (tried upto 300mM) 5mM BME 1mM pmsf 10% glycerol. Even it is also aggregating after NI -NTA purification but I am diluting protein then it...

31 December 2013 7,925 5 View

Can anybody explain how to design primers for a mature miRNA ?

I want to check the miRNA levels and miRNA transcript is short in length. How to design primer for a specific mature miRNA?

30 December 2013 1,020 6 View

Can anybody give me protocol for mitochondria staining using mitotracker?

I want to stain the mitochondria in suspension cells by using mitotracker red (Invitrogen). Can anybody explain protocol to observe them in fluorescence microscope?

19 December 2013 7,012 11 View

Can anyone explain why my MTT assay is giving off a brown colour instead of purple?

When I perform an MTT assay after adding DMSO, only a brown colour is appearing. I am working with suspension cells and I want to know the cytotoxicity of a compound. After 24 hours of seeding...

16 December 2013 4,526 14 View

Can anyone please recommend a protocol for analysis of surface expression of a CD marker using flow cytometry?

When I am performing an experiment I could not observe the any signal. Usually I use FITC tagged antibody but I am suspecting that I have problem with sample preparation and cells may be damaging.

27 September 2013 8,462 13 View