What is your opinion of "emotional intelligence" as a modern management tool?

Dear friend, what is your opinion of "emotional intelligence" as a modern management tool? Are there positive practices in your countries and in your institutions with its study and implementation?

14 December 2020 3,867 90 View

Statistical power analysis and sample size required to conduct Latent Class Growth Analysis ?

Hi, I'd like to conduct Latent Class Growth Curve analysis in order to explore inter-individual differences in the evolution of my variable. How could I estimate the a priori sample size...

27 November 2020 5,057 9 View

Same convergence indices and covariances using lavaan ML or MLR estimator?

Hi, I run CFAs with the lavaan package under R and I use the MLR estimator that fits the characteristics of my data. When I use the "summary(fit)" command, the fit indices for the MLR estimator...

18 November 2020 652 3 View

Is there a database of photos with insect pollinators on flowers of plants?

I would like to know if there is a database of photos with insect pollinators on flowers. The insects must be located on flowers of plants. I am interested in pollinators encountered in Europe,...

04 November 2020 2,219 6 View

Conducting CFA ith non normal data?

Hi, I'm looking for recommandations/articles to run Confirmatory Factor Analysis with non normal data. I've already used the WLS estimator using RStudio. Does anybody know methods for...

21 October 2020 4,340 3 View

How to run and evaluate Bayesian CFA model fit using R ?

Hi, Does anyboby know an R tutorial to run a Bayesian CFA and recommandations concerning the model fit evaluation and adjustment indexes of this type of analysis? I need to run a Bayesian CFA...

06 October 2020 5,819 2 View

How to run ESEM in R to evaluate structural validity of a questionnaire?

Hi, In order to validate psychometric properties of a questionnaire and more precisely the structural validity, the esem package :...

26 August 2020 4,397 3 View

What is the best European Table for food's amino acid composition ?

Hello dear network, I'm looking for a European composition table (if possible) to know the amino acid content of food. Do you have any ideas? Thanks you !

12 July 2020 5,494 2 View

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My colleagues study plant-pollinator networks. Insect (pollinator) identification is a task that requires a lot of effort. I would like to know if there are already good enough trained or...

21 April 2020 5,410 1 View

How is it possible to measure a electrical current created by an electron beam on an aluminium volume?

Dear , I am writing regarding GATE. Indeed, I wish to find a solution of the following problem: I realise, on GATE, the bombardment of electrons on an aluminium volume and I wish to collect...

24 March 2020 2,471 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View