We add zeatin, zeatin riboside and IAA only to the autoclaved medium, sterile, at 50C.
IBA, NAA, BP can tolerate autoclaving. but IAA , 2iP Can not tolerate autoclaving. Zeatin Picloram CCC do not know
2ip, BA, NAA , IBA and Picloram are stable. Zeatin and IAA are heat labile. I do not know about CCC
Actually it is not the question if one hormone is stable to heat, as you mean : safe for autoclaving. If you have a special hormone in mind, just trythe medium with the hormone autoclaved with the medium and and next to it the medium autoclaved and later adding the hormone at 50C. Do some tubes, add tissue and compare the response! Maybe even it is not so bad that the autoclaving cleaves the hormone, maybe the pieces of the hormones are effective too .
The table shown in this page might be useful as reference:
http://www.sigmaaldrich.com/life-science/molecular-biology/plant-biotechnology/tissue-culture-protocols/growth-regulators.html
NAA, IBA and BA are sensitive to temperature.I usually add IBA, NAA and BA after autoclaving to the autoclaved medium(50 C).
Gibberellins are rapidly degraded by elevated temperatures,
and the biological activity of a freshly prepared solution of GAj was
reduced by more than 90% as a result of autoclaving . The auxins NAA, IAA,
and 2,4-D are thermostabie. AlthoughIAA is unaffected by autoclaving under normal conditions, it is subjectto decomposition by other factors found under in vitro conditions.
For better results you can add the millipore filtered (whatman filter paper) PGR solution (Use autoclaved millipore water for preparing stock solutions of PGR'S) after autoclaving the media and then add according to the proportionate concentration required. (because these PGR's may lose activity after autoclaving Picloram, NAA, IAA, IBA)
Mostly we add after boiling the media and before poring in test tubes. yes autoclave effects its performance, but if we add it after autoclave, contamination control is more big issue.
All the PGRs mentioned by you such as IBA, NAA, BP can tolerate heating produced by autoclaving. but IAA , 2iP Can not tolerate autoclaving.
Adding PGRs after autoclaving is better for good results. BA, NAA, IBA 2,4 D can be added before autoclave with no big deal in efficiency. However, Zeatin, TDZ , IAA can perform better if added after autoclave.
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