For fine mapping in a QTL (with several genes) you can use a phenotyped progeny. First of all, you need both parental and descendants with extreme phenotype. For example, descendants with lowest and highest yield. We expect that descendants with extreme phenotype are homozygous in the QTL. You design markers along the QTL and you can deduced which allele is ligated with the phenotype. If you genotype along the QTL all the progeny, you will find recombinants (phenotype and genotype discordant) and you reduced the region (phenotype and genotype concordant) with the candidate gene.

I am working in this way for some years. I hope I help you with my explanation.

For fine mapping in a QTL (with several genes) you can use a phenotyped progeny. First of all, you need both parental and descendants with extreme phenotype. For example, descendants with lowest and highest yield. We expect that descendants with extreme phenotype are homozygous in the QTL. You design markers along the QTL and you can deduced which allele is ligated with the phenotype. If you genotype along the QTL all the progeny, you will find recombinants (phenotype and genotype discordant) and you reduced the region (phenotype and genotype concordant) with the candidate gene.

I am working in this way for some years. I hope I help you with my explanation.

QTL Mapping involving phenotypic and marker data and based on one of the several types of crosses derived from a parental cross between pure lines is used via software MAPMAKER/QTL to test the presence of QTL and estimate its position on the genome using a likelihood curve.

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