Atul Sathe

5 Questions 2 Answers 0 Followers

Questions related from Atul Sathe

How to perform semiquantitative-RT PCR to detect the abundance of alleles differing at single base (SNP)?

I'm working on a Rice mutant (spotted-leaf mutant) in which there is a SNP in the coding region of a gene. We are considering that the mutant allele is semidominant. We have T1 complementation...

07 July 2018 4,159 4 View

How to get the correct transcript out of alternatively spliced transcripts?

I'm trying to clone the CDS of a gene. This gene harbors 12 Exons and 11 Introns. Size of the CDS is about 4 kb. After amplifying the CDS, I have cloned it into a cloning vector. Sequencing result...

08 August 2017 8,959 11 View

What is the difference between fine mapping of a gene in mutants and QTL mapping?

In case of fine mapping of a gene in mutants, it is comparatively easy to find the location of a gene responsible for a trait. But in case of QTL mapping say for example for trait like yield in...

04 April 2016 7,654 5 View

How to reduce or eliminate the non-specific amplification in PCR?

I'm using the gene specific primer for screening the plant DNA samples. At the specific annealing temp (55) a 500 bp amplicon is getting amplified but there are significant non-specific bands too....

06 June 2014 6,813 13 View

Can any one tell me which are the heat stable plant tissue culture hormones and which are all the heat labile ones?

2-iP, Zeatin, BA, Picloram, NAA, IAA, IBA, CCC, etc.

11 November 2013 6,540 12 View