Total tests displayed by https://www.worldometers.info/coronavirus/#countries include rapid tests also or are solely RT-PCR Tests?

COVID-19 CORONAVIRUS PANDEMIC (https://www.worldometers.info/coronavirus/#countries) displays the total tests conducted countrywise everyday. Is this number based solely on RT-PCR tests or it...

30 October 2020 2,248 3 View

Hello everyone, good day! In the process of deproteinization of samples to obtain chitin, can I make use of a phenol as a deproteinizing agent?

I would like to know what other reagents can be used to obtain chitin by deproteinizing a sample. Commonly found on articles is the employment of NaOH concentrations as well as KOH. I would like...

16 October 2020 537 6 View

How can variables be clustered in few dimensions?

30 participants have given 50 adjectives (maximum 3 adjectives from each participants) for a phenomena. Now I want to to club/cluster these 50 different adjectives in fewer (say 8-10) adjectives....

24 September 2020 9,889 5 View

Which database should I use for systematic review?

If one one wants to conduct a systematic review in the field of psychology and specifically in cognitive functioning what will be the valid database, acceptable to reputed journal?

27 July 2020 6,812 4 View

In the paper mentioned can you help me to find the finite difference of equation no 19.?

In the paper "Magnetometer-Only Attitude Determination Using Novel Two-Step Kalman Filter Approach" in equation 19 do I need to complete quaternion multiplication first and then do simple...

15 June 2020 7,522 4 View

Is it better to upload a manuscript in the pre-print repository before published in the journal? Have you ever cited any pre-print articles??

Nowadays many researchers are uploading pre-print articles with designated DOI numbers and these are not peer-reviewed. During the peer-review process, the article could undergo major changes. If...

01 June 2020 4,006 90 View

Do predatory journals affect the standard and growth of science?

If the answer is yes, how to identify such type of publication houses? As these corrupted journals misuse the open access (OA) model by demanding a huge amount of money as article processing...

20 May 2020 5,764 12 View

Which reference management software/tool is best?

I am using Zotero and Mendeley reference management tools. Is there any other software which is better than these software and available free???

19 May 2020 6,721 46 View

ANN/Deep Learning/CNN etc., are they not overhyped?

ANN/Deep Learning/CNN etc., are they not overhyped? Today under the sun they promise to deliver solution to every problem. But in practise they have limitations. Besides this they are not...

27 December 2019 3,921 6 View

What is the Time Complexity of Multiway Split, Multi-Class Decision tree?

Decision Trees are extremely fast in classification. However, they are slow in construction during learning phase. Is there any paper on complexity analysis of Multiway Split, Multi-Class Decision...

12 November 2019 8,327 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

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17 February 2021 4,217 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How can I spin a thick layer of organic polymer?

I need to spin a 120um thick film. My current procedure consists of spinning the polymer (about 20um), uv exposure, plasma etch, and then spinning another 20um thick layer, uv again, plasma etch...

10 February 2021 2,055 6 View

What is the general miRNA yield one gets from the Qiagen miRNEASY serum/plasma advanced kit?

I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...

02 February 2021 8,806 2 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View