Can any one tell how to fix 6 dpf zebrafish larvae?

I am working on hepatoprotective activity. As a part of my study I have to conduct histopathological study of larvae. I went through different research papers. But each paper showing different...

02 March 2018 8,602 4 View

How to fertilize zebra fish eggs?

Dear all, I have collected zebrafish eggs after overnight mating of male with female zebrafish. But when I have collected these eggs and incubated in E3 medium for 24 hrs and observed under the...

05 June 2017 3,340 2 View

Although benzene as a solvent is carcinogenic, How if benzene extract is used for studying invivo activity?

I did invitro study of various herbal extracts. Result of invitro study showed maximum effect to benzene extract and hence I want to confirm the result by using invivo study. But literature...

10 November 2016 4,016 7 View

How if I am using combination of extracts for the study of its effect in early diabetic complications?

I have studied 15 solvent extracts of 3 different plants. In vitro study showed maximum anti diabetic activity to one of the plant extract (A) and maximum antioxidant activity to another plant...

07 August 2016 8,368 7 View

Can any one explain how to dissolve solid form of alpha amylase enzyme?

I am studying alpha amylase inhibitory activity of some plant extracts by using DNS color reagent. While performing bioassay I have observed that the solid form of enzyme after putting into 0.02...

05 June 2016 4,561 2 View

What is significance of flavonoid and phenolic content ratio? is there any correlation between flavonoids/ phenolic ratio and antioxidant activity?

I am taking five extract with different solvent for three plants. I did the study of flavonoid and phenolic content of 15 extracts (five extract of each plant). I want to select one or two extract...

03 April 2016 5,876 6 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

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02 March 2021 3,060 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View