I mean invitro models for study of antidiabetic activity except cell line or animal tissue
I am working on hepatoprotective activity. As a part of my study I have to conduct histopathological study of larvae. I went through different research papers. But each paper showing different...
02 March 2018 8,695 4 View
Dear all, I have collected zebrafish eggs after overnight mating of male with female zebrafish. But when I have collected these eggs and incubated in E3 medium for 24 hrs and observed under the...
05 June 2017 3,422 2 View
I did invitro study of various herbal extracts. Result of invitro study showed maximum effect to benzene extract and hence I want to confirm the result by using invivo study. But literature...
10 November 2016 4,087 7 View
I have studied 15 solvent extracts of 3 different plants. In vitro study showed maximum anti diabetic activity to one of the plant extract (A) and maximum antioxidant activity to another plant...
07 August 2016 8,450 7 View
I am studying alpha amylase inhibitory activity of some plant extracts by using DNS color reagent. While performing bioassay I have observed that the solid form of enzyme after putting into 0.02...
05 June 2016 4,646 2 View
I am taking five extract with different solvent for three plants. I did the study of flavonoid and phenolic content of 15 extracts (five extract of each plant). I want to select one or two extract...
03 April 2016 5,948 6 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View