I am studying alpha amylase inhibitory activity of some plant extracts by using DNS color reagent. While performing bioassay I have observed that the solid form of enzyme after putting into 0.02 mM phosphate buffer (containing 0.006 mM NaCL) pH 6.9 does not showed clear solution. It indicate that it is not completely dissolved in the buffer. Is there any other way to prepare alpha amylase solution?
and also let me know that once we prepare the enzyme solution is it required to maintain cold temperature?
Increase the salt concentraton. I think you mean 0..02 and 0.006 M, not mM. Anyway, NaCl can be increased to around 50 mM. In my hands, alpha-amylase is not very stable even frozen or kept at 4ºC. I am afraid you need to prepare fresh solutions. You have to check.
Dear Sir as per your suggestion I have tried increasing the concentration of NaCl but it did not work! Is there any other solution for the same? Thanks!
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