Hi,

You could use either Primer-BLAST (http://www.ncbi.nlm.nih.gov/tools/primer-blast/) or primersearch from EMBOSS package.

-Antti

if there are available and a validated pcr procedure this will be better 

but if not so you have to design the primer and validate the pcr procedure

good luck

Hi Sahil,

you can use In silico simulation of molecular biology experiments (insilico.ehu.es)

San Millán RM, Martínez-Ballesteros I, Rementeria A, Garaizar J, Bikandi J. 2013. Online exercise for the design and simulation of PCR and PCR-RFLP experiments. BMC Research Notes 6:513.

DOI: 10.1186/1756-0500-6-513.

or

Bikandi, J., San Millán, R., Rementeria, A., and Garaizar, J. 2004. In silico analysis of complete bacterial genomes: PCR, AFLP-PCR, and endonuclease restriction. Bioinformatics 20:798-9.

DOI: 10.1093/bioinformatics/btg491