What should the concentration of primers be when I am doing multiplex PCR?

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No title

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Nadine A. Gund

Hi, I think this will help you: http://medicine.yale.edu/labs/henegariu/www/tavi/p05.html

Cheers, Nadine

Budak Drop out

Download this article

Application of less primer method to multiplex PCR

Multiplex PCR design strategy used for the simultaneous amplification

of 10 Y chromosome short tandem repeat (STR) loci

http://cstl.nist.gov/strbase/pub_pres/Schoske2003.pdf

Multiplex PCR: Critical Parameters and

Step-by-Step Protocol

http://www.biotechniques.com/multimedia/archive/00011/97233rr01_11727a.pdf

Look at this article also.

Sumangala R C

3pmole forwardprimer and 1.5 pmole each of reverse primer should be fine. I usually use 3pmole concentration for multiplex PCR