I am using a single forward primer and two reverse primers.
Hi, I think this will help you: http://medicine.yale.edu/labs/henegariu/www/tavi/p05.html
Cheers, Nadine
Download this article
Application of less primer method to multiplex PCR
Multiplex PCR design strategy used for the simultaneous amplification
of 10 Y chromosome short tandem repeat (STR) loci
http://cstl.nist.gov/strbase/pub_pres/Schoske2003.pdf
Multiplex PCR: Critical Parameters and
Step-by-Step Protocol
http://www.biotechniques.com/multimedia/archive/00011/97233rr01_11727a.pdf
Look at this article also.
3pmole forwardprimer and 1.5 pmole each of reverse primer should be fine. I usually use 3pmole concentration for multiplex PCR
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