Sahil Vallabhbhai Patel

6 Questions 17 Answers 0 Followers

Questions related from Sahil Vallabhbhai Patel

Can Any one help me on In Silico PCR.??

Should i design new primers or available primers will do..

10 October 2014 7,897 3 View

How to get rid of the smear effect in my gel?

I am using multiplex PCR of genomic DNA in groundnut. One is an allele specific primer and the other is a universal primer.

05 May 2013 9,132 22 View

Ligation Procedure?

What is the reason for keeping the ligation reaction at 4 degree or 16 degree. I am using Quigen ligation kit. Why do we keep at different temperatures?

05 May 2013 10,068 7 View

What should the concentration of primers be when I am doing multiplex PCR?

I am using a single forward primer and two reverse primers.

04 April 2013 932 5 View

What problems could occur if the concentration of DNA in a PCR reaction is more than 200 ng?

What should be the amount of DNA in PCR reaction?

04 April 2013 5,281 5 View

How can we reduce or nullify the Smiling Effect in Polyacrylamide gels ?

The smiling that occurs when running a gel at higher voltage. it occurs at both ends and the bands form a smile.

04 April 2013 3,608 8 View