How helpful these bio reactors are in cell culture labs ???
The bioreactor works as a dynamic 3D cell culture condition. So, the cells spread out and penetrate through top to bottom of the bioreactor.
Can i have any reference to study about this ? kindly do refer some websites explaining its working and material description ..!! If u know any.. :)
The flow perfusion bioreactor stimulates by fluid shear stress the cells seeded on 3D system and provide enhanced delivery of oxygen, nutrients and growth factors throughtout the internal structure of culture scaffold
Thanks Mr. Teja for the pdf. Can i have some more application oriented bio-reactors?? Pls... !!
As Annalia pointed out, specific applications for bioreactors are primarily to solve a nutrient diffusion problem. They are extensively used in orthopaedic tissue engineering for example:
Cartlage : http://www.springerlink.com/content/w325758016841m02/
Bone : http://www.sciencedirect.com/science/article/pii/S8756328210016042
Then there is the demand for large cell populations at a high density which imposes mass transport limitations:
General problems: http://onlinelibrary.wiley.com/doi/10.1002/adma.200802748/abstract
Industrial scale-up: http://onlinelibrary.wiley.com/doi/10.1002/bit.22287/abstract
We constructed a bioreactor to grow trachea lined with epithelial cells on the inside and chondrocytes from the outside on cadaver decellularised trachea scaffold (it's a hollow tube). Bioreactor's role was to provide two diffferent media for two different cell cultures, maintain air/media level (trachea submerged lenghtwise to half in media, half in air). Trachea was rotated at 2rpm. here is a short video: http://juro.smugmug.com/OK-Otorhinolaryngology/Trachea-bioengineering/bioreactor-rotate-derail/1183056904_kb8jJ-S.jpg
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