Can any body tell me what is the sustitute for para nitro phenyl palmitate(p-NPP) ?

01 March 2014 1 7K Report

We are trying to do lipase assay(lipase source-Lactobacillus) using spectrophotometric method, so can any one suggest us any other alternative for p-NPP ?

Mohammad Vatanparast

You can also use of following substrates:

1- p-nitrophenyl butyrate(p-n-butyrate)

2- p-n- laurate

3- p-n-caprate

4- p-n-caproate

5- p-n-myristate

6- p-n-stearate

7- p-n-acetate

read absorbance at 405nm

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