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Questions related from Mohammad Vatanparast
I want to know for dsRNA synthesis what kind of vectors is proper.
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If the concentration of ethidium bromide is very high, what will have effects on visibility of bonds production on agarose gel. What is the best alternative for ethidium bromide?
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Since the dsRNA triggers the RNAi machinery, What principles should be considered in gene silencing for dsRNA prepartion? Which region of target gene should be considered to produce dsRNA?
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We have a column for gel filtration chromatography (120 cm*1cm) that we will use for enzyme purification. We want to load it by Sephadex G-75. What height (cm) of column packed with sephadex is...
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What concentration of protein(mg/ml) is appropriate for gel filtration(Sephadex G-75) and ion exchange (DEAE-cellulose) chromatography? (Gel filtration column (120*1.5cm) and ion exchange column...
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