Can any body help me?

06 June 2017 0 2K Report

I have five primers with Tm range 55, 56, 56.8, 57.7. I did run on all these specific annealing temperature, but yet i got no result. I am working with IL6, TNF, VEGF, CYP4BI and GADPH. I have optimized just GADPH. What should i do now?

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Science method

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