Can any body help me?

I have five primers with Tm range 55, 56, 56.8, 57.7. I did run on all these specific annealing temperature, but yet i got no result. I am working with IL6, TNF, VEGF, CYP4BI and GADPH. I have...

05 June 2017 1,748 0 View

I performed RNA extraction, concentration of my RNA was 603.22 but its 260/280 value is 2.91. which is very high. What should i do now?

TRIZOL reagent was used for RNA extraction.

02 March 2017 3,509 3 View

How can we find out the amplicon size of already known primer sequence? kindly guide me?

11 December 2016 6,404 1 View

What is this fern?

Please help me to identify this fern species. Thank you in advance.

24 February 2021 2,435 5 View

How to use Remote sensing and GIS applications for mapping the available medicinal plants within the locality/municipality?

Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...

21 February 2021 374 5 View

Can medicinal plants really play an inherent rule in the COVID-19 tackle?

Numerous scientific reports cited plant extracts with potential antiviral activity and even anti-COVID-19? Why these findings are neglected? I remember very well the warning released by the WHO to...

17 February 2021 2,141 9 View

Is there any solar electric power plant having the concentrated photovoltaic modules?

The solar photovoltaic (PV) power plant uses commercial (non-concentrated) PV modules which are quite simple in design and reliable. They can work on fixed orientation and don't need the moving...

16 February 2021 3,796 9 View

Is it possible estimating environmental conditions with a Bayesian approach?

In ecology specific species can be used to get an indication/estimation of the environmental conditions. A simplistic example, lets assume I find a pike (Esox lucius). We know this species does...

01 February 2021 10,034 4 View

How to add journals not listed on Research Gate ?

I have two publications one listed in the web of science master list " European Online Journal of Natural and Social Sciences " ISSN: 1805-3602 and Second " Journal of Managerial Sciences"...

26 January 2021 7,158 7 View

Can I use MERRA-2 model's "500hpa geopotential height" monthly data in excel form for districts or I download the data in netcdf files and extract it?

I want to use MERRA-2 model's monthly data of 500hpa geopotential height for different districts of Pakistan (1980-2019). I want to download this data from NASA Giovanni and I want to select time...

25 January 2021 6,144 1 View

How can a traditional medicine exist if its validated outcome is translated into molecular mechanism for acceptance of modern medicine?

A traditional medicine (e.g. Unani System of Medicine) has its own philosophy of treatment of a disease. Nowadays, there is emerging demand to validate the effectiveness of an intervention (a...

05 January 2021 9,472 5 View

Why people feel fear and not share their particulars during social or questionnaire survey, especially in Pakistan?

Why people feel fear and not share their particulars during social or questionnaire survey, especially in Pakistan?

01 January 2021 4,782 12 View

How yo prepare samples for RNA extraction?

I really need help. I tried finding a protocol on how to prepare samples for gene expression . I am working on Antimicrobial activity of a medicinal plant and I would like to check its effect on...

30 December 2020 7,112 2 View